Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Novel Whole Genome Amplification For Difficult And Limited Templates

Chad T Brueck , Lukasz D Nosek , Clyde R Brown , Ernest J Mueller , Rafael Valdes-Camin , Kevin J Kayser

  Sigma-Aldrich Biotechnology 2909 Laclede Ave Saint Louis, MO, 63103, USA

Multiple scientific disciplines rely on molecular biological analyses at the genome-wide scale. This research requires a significant amount of high quality DNA. Certain samples, such as formalin fixed paraffin embedded (FFPE) tissues and single cells, do not provide sufficient amount or quality of genomic material. To enable genomic analyses and genome immortalization, we report the development of a whole genome amplification (WGA) method suitable for amplification of DNA from challenging sources. This technology has been successfully used to amplify nanogram amounts of DNA resulting in microgram yields from multiple plant, soil, bacterial, FFPE tissue and animal sources. In addition, WGA has been adapted to provide the sensitivity to amplify entire single cell genomes from multiple human cell types and Arabidopsis thaliana cells. We present real time quantitative data for multiple sources, and representative single cell results.