Plant & Animal Genomes XIV Conference

January 14-18, 2006
Town & Country Convention Center
San Diego, CA

Nuclear DNA Amounts In 114 Brazilian Tree Species

Marcelo C Dornelas¹ , Tamara C Fonseca² , Fernanda G Pereira³ , Irene Lorand-Metze³

¹  Universidade Estadual de Campinas, Instituto de Biologia, Depto. Fisiologia Vegetal, Cidade Universitária ¨Zeferino Vaz¨, Barão Geraldo, 13084-971, Campinas, SP, Brazil

²  Instituto de Zootecnia, APTA-São Paulo, Centro de Pesquisa e Desenvolvimento em Zootecnia Diversificada, Rodovia Nova Odessa-Piracicaba, SN, Nova Odessa, SP, Brazil

³  Universidade Estadual de Campinas, Hemocentro, Lab. Marcadores Celulares, Cidade Universitária ¨Zeferino Vaz¨, Barão Geraldo, 13084-971, Campinas, SP, Brazil

The current information on nuclear DNA content of angiosperms is biased towards crop plants and their wild relatives. Aiming to improve geographical representation for angiosperm C-values, we have determined the nuclear DNA content of 114 Brazilian tree species. DNA content was determined by flow citometry. The mean genome size found was around 1pg. The largest 2C-value was found for Syagrus romanzoffiana, (Arecaceae, 2C=6.13pg). On the other hand, 2C=0.35pg for Tibouchina granulosa (Melastomataceae). We provide the first estimate for the nuclear DNA content for 108 out of the 114 Brazilian tree species studied. This is also the first report for 9 angiosperm families and 60 genera.