PAG-XIII (W040) The Gene eIF4E Is A Common Determinant Of Recessive Virus Resistance In Dicot And Monocot Plants As Revealed By Map-Based Cloning Of Rym4 Conferring Bymovirus Resistance In Barley

Plant & Animal Genomes XIII Conference

January 15-19, 2005
Town & Country Convention Center
San Diego, CA

W040 : Barley

The Gene eIF4E Is A Common Determinant Of Recessive Virus Resistance In Dicot And Monocot Plants As Revealed By Map-Based Cloning Of Rym4 Conferring Bymovirus Resistance In Barley

Nils Stein¹ , Dragan Perovic¹ , Bettina Pellio² , Jochen Kumlehn¹ , Thomas Wicker³ , Silke Stracke¹ , Frank Ordon^2,4 , Andreas Graner¹

¹ Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Gatersleben, Germany

² Institute of Crop Science and Plant Breeding I, Giessen University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany

³ Center for Applied Genetic Technology, University of Georgia, 111 Riverbend Road, Athens, GA 30602, USA

⁴ Federal Centre for Breeding Research on Cultivated Plants, Institute of Epidemiology and Resistance, Theodor-Roemer-Weg 4, 06449 Aschersleben, Germany

The yellow mosaic disease in barley is caused by different isolates of the bymoviruses BaMMV and BaYMV (family Potiviridae). Several independent and mostly recessive resistance genes have been localised on at least 5 independent loci within the barley genome and provide the genetic basis for a control of the disease. A 600 kb contig was established by map-based cloning partially co-segregating with the Rym4/5 locus on barley chromosome 3HL in over 5,000 meiotic events. Full-length sequence analysis of 439 kb of the contig led to the identification of only two genes and a complex arrangement of over 88% of repetitive DNA. One gene is Hv-eIF4E. Orthologs of this gene have been shown previously to be involved in Potyvirus resistance in pepper, lettuce, Arabidopsis and melon but so far not in any monocot species. A number of rym4/5-diagnostic SNPs has been detected in four exons of the gene all effecting AA-changes. Complementation via stable transformation of a resistant barley cultivar with putative susceptibility alleles led to the induction of susceptibility and therefore confirmed the role of the gene in bymovirus resistance in barley.