Plant & Animal Genomes XIII Conference

January 15-19, 2005
Town & Country Convention Center
San Diego, CA

The Inflammatory Response In The Rainbow Trout (Oncorhynchus mykiss) Liver: A Microarray Analysis.

Lena Gerwick , Graham Corley-Smith , Susan C. Tilton , David E. Williams , Christopher J. Bayne

  1.Department of Zoology, Oregon State University, Corvallis, Oregon 97331, 2. Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon, 97331, 3. Marine Freshwater Biomedical Center, Oregon State University, Corvallis, Oregon, 97331

When earlier examined using gene-by-gene approaches, the inflammatory response in Rainbow trout (Oncorhynchus mykiss) liver resembled that of higher vertebrates. We have now completed a broader analysis of the response using a custom-made DNA microarray. Trout were injected with killed Listonella anguillarum. Total RNAs from liver tissue collected 3, 12 and 24 hours post treatment from non injected and injected trout were reverse transcribed and subsequently hybridized to the 70mer oligonucleotide (OSU trout v2.0) DNA array developed in our laboratory. This array contains ~1500 trout genes involved in immunological, toxicological and endocrine responses. When the OSU microarray and a salmonid cDNA array (GRASP) were hybridized to identical cDNA preparations from trout treated with 17â Estradiol and 3,3 diindolylmethane, transcriptional pattern changes were generally as expected, and consistent between platforms. Genes with significant up-regulation after the bacterial injections included isoforms of precerebellin-like protein, isoforms of complement component 3, trout C-polysaccharide binding protein, haptoglobin, CD59 and chemotaxin.
* Corresponding author; gerwickl@science.oregonstate.edu