Plant & Animal Genomes XIII Conference Plant & Animal Genomes XIII ConferenceJanuary 15-19, 2005
Town & Country Convention Center
San Diego, CA
Jill C. Graff , Michael Behnke , Jay Radke , Michael White , Dustin Lee , Mark A. Jutila
Gamma/delta T cells represent only a minor population of T lymphocytes in most animals, but their importance within the immune system is expected due to their strong evolutionary conservation. Due to the lack of a broad knowledge-base of gamma/delta T cells, global analyses of gamma/delta T cell transcriptomes have been performed using serial analysis of gene expression (SAGE). New SAGE libraries of resting and Con-A/IL-2 activated splenic and blood gamma/delta T cells, and resting and Con-A/IL-2 activated alpha/beta T cell-enriched splenocytes were generated to complement earlier SAGE libraries of circulating tissue-specific gamma/delta T cell subsets, yielding a total of 9 SAGE libraries that could be compared. These databases have been analyzed and compared using new WEB-page based bioinformatic tools, allowing the user to rapidly compare gene expression patterns within these and other SAGE and standard EST libraries generated from different cell types and different species. Unique subset- and tissue-specific gene expression patterns have been identified, which define common, as well as distinct, functional profiles of alpha/beta and gamma/delta T cells. These databases provide additional resources for the annotation/analysis of any global gene expression determination in bovine immune cells. Funded by a grant from the USDA IFAFS program.