Plant & Animal Genomes XIII Conference Plant & Animal Genomes XIII ConferenceJanuary 15-19, 2005
Town & Country Convention Center
San Diego, CA
Tomoko Eguchi-Ogawa , Takeya Morozumi , Kohei Suzuki , Takashi Awata , Hirohide Uenishi
T lymphocytes recognize wide variety of antigens including peptides, glycolipids and phosphorylated metabolites presented by MHC or related molecules. It has been revealed that lipid antigens such as lipopeptides of mycobacteria and marine sponge-derived glycolipid (a-galactosylceramide) are recognized by human and murine TCRs in the context of CD1 molecules. To date, five CD1 genes have been identified (CD1A, CD1B, CD1C, CD1D and CD1E) in humans, whereas only a CD1A ortholog, CD1.1, has been ascertained in pig. From our porcine 5’-EST database based on full-length cDNA libraries (PEDE), we newly identified porcine CD1 genes (pCD1B, pCD1D and pCD1E) in addition to CD1.1. We constructed a BAC contig to clarify genomic structure of the porcine CD1 gene cluster. By shotgun sequencing and sequence analysis, it was revealed that the cluster of porcine CD1 genes was located between KIRREL and olfactory receptor genes, like human. However, CD1.1 and CD1C orthologs could not be found in the region, and another CD1A ortholog that we could not observe its expression was detected. Furthermore, the pCD1B gene was detected in opposite transcriptional direction to that of human. The analysis of genomic and cDNA sequences of porcine CD1 families presented here contributes to analysis of varied host options in immune response to diverse spectra of pathogens. (This work was supported by MAFF, Japan and JRA.)