Plant & Animal Genomes XIII Conference

January 15-19, 2005
Town & Country Convention Center
San Diego, CA

Use Of Marker Assisted Selection In Identifying Hessian Fly Resistance In Segregating Populations.

Justin Wheeler¹ , Dennis J. Schotzko² , Nilsa A. Bosque-Pérez² , Mary J. Guttieri¹ , Edward J. Souza¹

¹  University of Idaho 1693 S 2700 W Aberdeen, ID 83210

²  University of Idaho PSES Department Moscow, ID 83843

In wheat (Triticum aestivum, L.), the H25 gene confers resistance to most biotypes of Hessian fly (Mayetiola destructor Say). The wheat germplasm ‘KS92WGRC20’ is a source of the H25 resistance gene and has been used to introduce resistance to adapted wheat breeding lines. Eighty F2 plants of the population derived from the cross IDO599/IDO584 (IDO584 contains the H25 gene from KS92WGRC20) were infested with Hessian fly adults in the laboratory to evaluate reaction to the pest. Phenotypic data was collected for plant height, number of tillers, number of larvae, and number of puparia. Microsatallite markers that map to the same chromosome arm as the rye translocation carrying H25 gene were used to map Hessian fly reaction. The marker Xgwm397 on chromosome arm 4AL is linked to H25 and correlates closely to the number of larvae and puparium. Segregation studies involving the above F2 lines segregating for H25 confirmed a second marker, Xgwm610, more tightly linked than Xgwm397. Despite low frequencies of recombination between the markers and the H25 gene, these markers are valuable as tools in identifying Hessian fly resistance in segregating populations.