Plant & Animal Genomes XIII Conference

January 15-19, 2005
Town & Country Convention Center
San Diego, CA

Preliminary QTL Analysis Of Dwarf Bunt And Stripe Rust Resistance In A Winter Wheat Population

Paul St Amand¹ , Mary J. Guttieri² , David Hole⁴ , Xianming Chen³ , Gina Brown-Guediera¹ , Edward J. Souza²

¹  Kansas State University Agronomy Dept. 2004 Throckmorton Hall Manhattan KS 66506-5501

²  University of Idaho P.O. Box 870 1693 S. 2700 W. Aberdeen, ID 83210

³  USDA-ARS Box 646420, 209 Johnson Hall, WSU Pullman, WA 99164-6420

⁴  Utah State University Ag Sci Bldg 334 Logan UT 84321

A preliminary genetic map of a recombinant inbred line (RIL) winter wheat (Triticum aestivum L.) population (Rio Blanco/IDO444) was constructed to begin QTL analysis of dwarf bunt (Tilletia controversa) and stripe rust (Puccinia striiformis f. sp. tritici) resistance data. Dwarf bunt disease evaluation was conducted at three locations in Utah and Idaho in 2003 and 2004. RIL stripe rust resistance was evaluated at Mt. Vernon and Pullman, WA in 2003 and 2004. Evaluation of 296 RIL suggests the presence of 5 or more dwarf bunt and 3 or more stripe rust resistance genes. A total of 401 SSR primers amplified scorable fragments, and 159 were scored as polymorphic between the parents. The number of polymorphisms per chromosome ranged from 3 markers each for chromosomes 1A, 1D and 4A to 13 for chromosomes 2D and 6D. No markers on chromosome 6B tested thus far were polymorphic between the parents. All polymorphic markers were evaluated on a subset of 96 RIL. Nineteen linkage groups were formed from the polymorphic markers. QTL analysis identified dwarf bunt resistance linked to DNA fragments on the group 3 chromosomes, including fragments amplified by SSR primers gwm114 and BARC8. Stripe rust resistance was linked in the population to DNA fragments amplified by SSR primers gwm120, gwm148, and BARC91. These fragments were previously mapped to chromosome 2B and the QTL for resistance is likely on the 2BS arm.