Plant & Animal Genomes XIII
Conference Plant & Animal Genomes XIII
ConferenceJanuary 15-19, 2005
Town & Country Convention Center
San Diego, CA
The Life Sciences Group of Brooks Automation, Inc. has developed a fully automated, integrated platform to
perform nanoliter volume cycle sequencing reactions. Our standard DNA cycle sequencing reaction is performed in a total volume
of 500 nanoliters, which is 98% smaller than a standard reaction. A key element in the Parallab 350™ is the proprietary
Nano-Pipetter. This innovative device utilizes 96 glass tubes to perform all of the reaction processing steps, including:
assembling, mixing, thermal cycling, and magnetic bead purification. After each sample set is completed, the Nano-Pipetter is
cleaned and reused, greatly reducing the amount and cost of consumables normally associated with conventional processing methods.
In this presentation, we discuss sequencing data from a wide range of conditions. The Parallab 350 produces quality sequencing
data from plasmids, PCR products, BAC’s and TempliPhi™. Because the reactions are miniaturized, not diluted, we have
proven success with a 16-fold concentration range of input template. We also show a reduction in Big Dye® reagent used to
160th of a standard 1x reaction. In addition, integrated tools for magnetic bead dye terminator removal allow the automation of
multiple commercially available kits. This unique combination of attributes (nanoliter volume reactions, reduced consumables,
and complete automation) enable researchers to easily integrate this highly novel platform into a variety of laboratory
environments, for a broad number of applications and immediately see cost and labor savings without compromising data quality.