Plant & Animal Genomes XIII Conference

January 15-19, 2005
Town & Country Convention Center
San Diego, CA

Resources For Genome Study From The Arizona Genomics Institute BAC/EST Resource Center

David Kudrna , Chris Mueller , Mei-Zhong Luo , Jetty Ammiraju , Nick Sisneros , Jayson Talag , Kiran Rao , Elizabeth Ashley , Angelina Angelova , Hye-Ran Kim , Yeisoo Yu , Cari Soderlund , Rod Wing

  Arizona Genomics Institute and Computational Laboratories, Department of Plant Sciences and Bio5, University of Arizona, Tucson AZ 85721 USA

The Arizona Genomics Institute BAC/EST Resource Center provides affordable access to BAC and cDNA libraries, high-density hybridization filters and clones for the world wide genomics community. The Center serves as a primary platform to launch our BAC library construction, physical mapping, genome and EST sequencing and positional cloning projects. Biological resources from these projects are available to the public from our on-line ordering system at: http://genome.arizona.edu/orders/. We also accept clones from the scientific community to be archived and distributed world-wide as well as perform custom jobs.
The Center operates 2 Genetix Q-bots (colony picking, library replicating and rearrarying, filter gridding) and 44 -80 freezers (clone archiving) that provide high-throughput and barcode traceable clone handling capabilities. Recent BAC library projects include:
1) BAC library production and distribution for "Healthy People 2010" (NIH: PI Wing, Co-PIs Tomkins and Soderlund). Recently completed BAC libraries include: wallaby (Macropus eugenii, ME_KBa), zebra finch (Teaniopygia guttata, TG__Ba), nurse shark (Ginglymostoma cirratum, GC__Ba), rat (Rattus norveigicus, RN__Ba), Drosophila ananassae (DA__Ba), Drosophila mojavensis (DM_CBa), Drosophila virilis (DV_VBa), snail (Biomphalaria glabrata, BG_BBa) and echidna (Tachyglossus aculeatus, TA__Ba).
2) The Oryza BAC library project: A fundamental public resource to study monocot plant evolution, development and physiology (NSF: PI Wing, PI Co-PIs Soderlund, Tomkins, Jackson & Luo). Recently completed BAC libraries include: Oryza rufipogon(Nivara, annual), O. brachyantha, O. alta, O. officinalis, O. ridleyi, O. punctata, O. minuta, O. australiensis, O. coarctata, O. granulata, O. glaberrima, and O. rufipogon (perennial).
3) The Green Plant BAC Library Project (NSF: PI Mandoli, Co-PIs Wing, Soderlund, Tomkins, DePamphilis & Banks). Recently completed: BAC libraries for Volvox carteri (BAC, VCNEBa HindIII and VCNEBb BamHI), Acorus gramineus (AA__Ba), Nuphar advena(NA__Ba), and Selaginella moellendorfii (SM__Ba).