Plant & Animal Genomes XIII Conference

January 15-19, 2005
Town & Country Convention Center
San Diego, CA

Construction Of Arabidopsis Transcription Factor Orfeome For Genome Wide Study Of Protein-Protein Interactions Of Plant Transcription Factors

Hye Jin Kim , Young Hun Song , Su Young Shin , Ju Hwoan Kang , Na Young Song , Jong Chan Hong

  Department of Biochemistry, Division of Applied Life Science, Gyeongsang National University, Jinju, 660-701, Korea

Transcription factors (TFs) play a key role in various growth and developmental process of plant. The understanding the complex mechanism of transcription regulation requires genome-wide analysis of large number of transcription factor families. To verify the genome annotation and to create resource to functionally characterize the transcription factor open reading frames we attempted to clone all predicted protein-encoding reading frames of Arabidopsis thaliana transcription factor. We successfully cloned 1,220 Arabidopsis TF ORFs predicted by computer analysis. Recently Gong et al.(2004)) reported the isolation of 1,282 Arabidopsis TF clones. The combined ORFeome collection resulted in the isolation of 1,543 TF ORFs out of 1,904 TF ORFs predicted. These transcription factor ORFeome clones have been obtained in the Gateway entry vector (donor vector), a powerful recombinational cloning (RC) system. We have constructed expression library for yeast two-hybrid screen carrying 1,543 transcription factor ORFeome in correct reading frame. Our study shows that the newly generated yeast AD-ORFeome library will be a valuable tool in isolating many novel interacting proteins to the regulatory protein of interest (Supported by BK21 program and the grant from Crop Functional Genomic Center of 21C Frontier Research Program).