Plant & Animal Genomes XIII Conference

January 15-19, 2005
Town & Country Convention Center
San Diego, CA

ESTs Analysis Of Japanese Flounder (Paralichthys olivaceus) Head Kidney And Brain Cells Stimulated With Poly I:C

Ikuo Hirono , Praprudee Piyaviriyakul , Ikumi Haraguchi , Takashi Aoki

  Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo, 108-8477, Japan

Sequencing of cDNA libraries to generate large numbers of partial cDNA expressed sequence tags (ESTs) has been shown to be a powerful tool to identify homologues of reported genes of different animals and novel genes. Previously, we focused on isolation and identification of immune-related genes from Japanese flounder by EST analysis. We found many different immune-related genes from Japanese flounder. However, the number of our isolated immune-related genes is not enough for understanding the fish immune system at the molecular level. In this study, we constructed two different cDNA libraries of polyI:C stimulated kidney cells (JFK-PIC) and brain cells (JFB-PIC). We sequenced 1285 clones from JFK-PIC cDNA library and 599 clones from JFB-PIC cDNA library. Approximately 30 % of these ESTs have no homology with published DNA sequences. We found several newly isolated immune-related gene homologues such as CD2, CD59, CD97, CD109, TLR2, TKR inhibitor, CSF receptors, chemokine receptors, interferon-gamma-inducible protein-10, Lymphoid-specific interferon regulatory factor and so on. Some of cytokines and receptors were expressed in brain.