Plant & Animal Genomes XIII Conference Plant & Animal Genomes XIII ConferenceJanuary 15-19, 2005
Town & Country Convention Center
San Diego, CA
Bread wheat (Triticum aestivum) contains one of the largest genomes of the plant kingdom and is a significant challenge when considered for genome sequencing. Wheat is known to have gene-rich regions within the genome interspersed by vast amounts of repetitive DNA. It is understood that a filtering technique would be of significant benefit when targeting gene containing regions for sequencing. Previous work has indicated that the restriction enzyme, PstI can increase the presence of low copy DNA when used to digest genomic DNA. To evaluate this on a larger scale, genomic DNA of the bread wheat ‘Chinese Spring’ was digested with the methyl sensitive PstI. DNA fragments of 2 – 4 Kb were subcloned into cloning vector, isolated, and single pass sequenced. The project goal is to isolate and sequence more than 3,000 clones to get a sub sample of the capability of this technique. The sequencing results will be evaluated for the presence of open reading frames, repetitive DNA and low copy DNA. The results will be reported here.