Speakers: Recent Advances in DNA Microarray Analysis and Genomic DNA Cloning

3:30 PM - 4:30 PM

A new and improved RNA amplification process for DNA microarray analyses. DNA microarrays are an established and powerful technique for analyzing the transcriptional profile of plant and animal cells. Increasingly, RNA amplification methods are being employed to generate sufficient labeled target nucleic acid for use with microarrays when cellular mRNA is limiting (i.e. from a minute number of cells). We will introduce EPICENTRE's new TargetAmp? RNA Amplification kits which utilize an improved and simplified "Eberwine" RNA amplification procedure that is capable of producing microgram quantities of amplified RNA from the total RNA contained in as little as one cell (>5,000,000-fold amplification). We will present an overview of the TargetAmp RNA amplification process and provide supporting data and results.

4:40 PM - 5:40 PM

The use a of low-copy number vector can significantly improve the stability of DNA inserts, whether your working with large genomic fragments or 2-kb cDNAs. EPICENTRE's CopyControl? Cloning Systems provide the necessary reagents for constructing libraries of stable, single-copy BAC, fosmid or smaller-insert clones that have the added advantage of enabling the user to induce clones to higher copy numbers for higher yields of DNA. The relative merits of these systems will be discussed with special emphasis given to cloning efficiency, fidelity, and reliability. We will also introduce our new CopyCutter? E.coli cells which significantly lower the copy number of many commonly used vectors thereby simplifying the process of cloning "toxic" or unstable DNA sequences. New kits for the purification of BAC and fosmid DNA will also be presented with data validating their superiority with respect to yield and quality.

This page last updated Tuesday, 21-Dec-2004 20:28:33 EST