THE BRASSICA A GENOME PHYSICAL MAP AND COMPARATIVE ANALYSIS OF BRASSICA GENOME MICROSTRUCTURE

¹ John Innes Centre, Norwich, UK

² HRI, Wellesbourne, UK

³ University of Birmingham, UK

⁴ University of Bath, UK

⁵ Chungnam National University, Taejon, S. Korea

Full author list:
John Innes Centre, Norwich, U.K.: Debashis Rana, Tom van den Boogaart, Llewelyn Hynes, Stephane Riviere, Carmel O'Neill, Paul Beckett, Elisabeth Bent, Leah Clissold, Stephanie Langford, Lee Macpherson, Rachel Wells, Vicky Smith, Martin Trick and Ian Bancroft
HRI, Wellesbourne, U.K.: Guy Barker, Rowena Naylor, Dina Patel, Sarah Stevenson, Neale Grant, Carol Cave, Jim Beynon, Graham King
University of Birmingham, U.K.: Xiao-Y Wang, Mike Kearsey
University of Bath, U.K.: Andrea Dowling, Rod Scott
Chungnam National University, Taejon, S. Korea: Jee Young Park, Yong Pyo Lim

The cultivated Brassica species represent models both for the application in crops of genomic information gained in Arabidopsis and for the study of plant genome evolution. In order to extend investigations into the species containing the Brassica A genome, we have constructed a BIBAC library from genomic DNA of Brassica rapa subspecies trilocularis. The clones of this library were assembled, using a strategy involving both fingerprinting and systematic hybridisation, into a BAC contig-based physical map (http://brassica.bbsrc.ac.uk/IGF/?page=body/project.htm).

How useful is the A genome physical map, and the forthcoming A genome sequence, (http://brassica.bbsrc.ac.uk/brassica_genome_sequencing_concept.htm) going to be for the support of research and gene cloning in the other widely grown Brassica crop species: B. oleracea (C genome) and B. napus (containing both A and C genomes)? To begin to investigate this, we constructed a BAC library of genomic DNA of B. napus variety Tapidor and undertook a comparative analysis of the microstructure of homoeologous regions of the genomes of B. rapa, B. oleracea and B. napus.