Plant & Animal Genomes XII Conference

January 10-14, 2004
Town & Country Convention Center
San Diego, CA

Poster: Other Marker Related Topic
           PSID

CLOSE RELATIONSHIP BETWEEN CHOCOLATE COSMOS AND YELLOW COSMOS REVEALED BY PS-ID ANALYSIS FOLLOWED BY THE HYBRID PRODUCTION

¹ Graduate School of Science & Technology, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan

² Department of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan

Chocolate cosmos (Bidens atrosanguinea) has chocolate-color flowers with a strong scent of chocolate and leaves like miniature Dahlias. The chocolate cosmos, one of endangered flowering plants, has ambiguously been classified into Cosmos atrosanguineus or Bidens atrosanguinea. We have tried to resolve phylogenetic relationships of chocolate cosmos among the genus Cosmos, Bidens and Dahlia by analysis of plastid subtype identity (PS-ID) sequences. The PS-ID sequence show that chocolate cosmos has a close relationship with Cosmos species than Bidens and Dahlia. Based on PS-ID data, chocolate cosmos was hybridized with two Cosmos species, C. bipinnatus (common cosmos) and C. sulphurensis (yellow cosmos). Finally, we could produce a hybrid plant by crossing with C. sulphurensis and by rescue of embryo culture. The hybrid plant shows intermediate phenotype like crimson-red flower and the hybridity is also proven by DNA fingerprint and flow cytometry analysis.