Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Workshop: Cattle/Sheep
            

A FINGERPRINTED BAC CLONE PHYSICAL MAP OF THE BOVINE GENOME

¹ Genome Sciences Centre, B.C. Cancer Research Centre, 600 W.10th Ave, Vancouver, B.C., V5Z-4E6,Canada

² BACPAC Resources, Children's Hospital Oakland Research Institute, Oakland, 747 52nd St., CA, 94609, U.S.A.

³ Department of Animal Science, Texas A&M University, College Station, TX, 77843, U.S.A.

We have constructed a high-resolution BAC-based physical map of the bovine genome using an agarose gel-based fingerprinting methodology. The map consists of 294,652 large-insert BAC clones from three libraries, CHORI-240 (male Hereford), RPCI-42 (male Holstein) and TAM-BT (male Angus), fingerprinted with HindIII restriction enzyme. These fingerprints were assembled computationally into 8,498 contigs using FPC, a software package that detects overlapping clones based on similarities in their fingerprints. This map has been generated as part of an international consortium, and will be integrated with BAC end sequences, markers and additional mapping data.

Similar maps have been constructed for large mammalian genomes such as human, mouse and rat. These maps have contributed to the sequencing and analysis of those genomes by assisting in the selection of high quality clones, sequence finishing and verification of sequence assemblies. They have also been important tools for QTL discovery, the construction of transgenics and comparative genomics studies in many other organisms.

This fingerprint map will be a valuable resource for future bovine genetic and molecular research. The FPC fingerprint database and associated marker data are publicly available for download (http://www.bcgsc.ca) and may also be easily viewed with our Java-based program, iCE (Internet Contig Explorer, available at http://ice.bcgsc.ca/).