Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Workshop: Cattle/Sheep
            

DEVELOPMENT OF GENOMIC TOOLS, A BOVINE MICROSATELLITE-BASED LINKAGE MAP AND AN EST-RH MAP

¹ Shirakawa Institute of Animal Genetics, Fukushima, Japan

² University of Minnesota, Department of Veterinary PathoBiology, St. Paul, MN, USA

³ Cattle Breeding Development Institute Kagoshima, Kagoshima, Japan

⁴ National Livestock Breeding Center, Fukushima, Japan

⁵ USDA-ARS, U.S. Meat Animal Research Center, Clay Center, NE, USA

⁶ University of Nevada, Department of Animal Biotechnology, Reno, NV, USA

High-density genetic and physical maps are indispensable for positional cloning of the genes responsible for economic traits in livestock. A large number of microsatellites mapped on a genetic map are important for narrowing a critical region by linkage analysis as well as constructing a robust RH map. A precise EST-RH map is required to find a corresponding region and candidate genes on human and mouse genomes. Our aim is to develop such genomic tools i.e. a genetic map including 3000 microsatellites, and an RH map including 3000 ESTs in addition to microsatellites used as framework. We have isolated over 1500 microsatellites, and fine-mapped 612 of them and published 142 on the USDA-MARC cattle linkage map, resulting the map includes 2141 markers at 1.3 cM-interval on average. Also, we have prepared a 7000 rad bovine-hamster whole genome RH panel consisting of 92 hybrid clones, and placed over 1000 ESTs in the framework consisting of 1078 microsatellites localized on the USDA-MARC linkage map.