Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Workshop: Brassicas
            

BRASSICA OLERACEA GENOME STRUCTURE AND GENE CONTENT BASED UPON FINISHED HOMEOLOGOUS BAC CONTIGS AND WHOLE GENOME SHOTGUN SEQUENCES.

¹ The Institute for Genomic Research, 9712 Medical Center Drive, Rockville MD 20850, USA.

² The John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, U.K.

We are involved in two sequencing projects aimed at understanding the structure and gene content of the Brassica genome and its relationship to Arabidopsis. In a whole genome shotgun project, we have sequenced several small insert libraries prepared from the rapid-cycling doubled haploid Brassica oleracea line TO1000DH3 and have generated 411,693 good sequences with an average read length of 666 bases for a total of 274 Mb. Of these sequences, about 7, 4 and 3% are chloroplast, ribosomal RNA encoding and mitochondrial respectively. Approximately one quarter of the sequences match a previously reported protein. By further sequence analysis we expect to obtain some insight into other components of the Brassica genome such as repetitive elements and transposon-like sequences. In order to evaluate sequence variation across homeologous regions of the Brassica genome, we have begun to sequence BACs from 6 contigs representing a region of the genome that itself is part of an ancestral duplication present both in Brassica oleracea and Arabidopsis thaliana. Comparisons of genome organization and gene content in these regions both within and between species will be reported. This work was supported by the National Science Foundation.