Plant & Animal Genomes XI Conference Plant & Animal Genomes XI ConferenceJanuary 11-15, 2003
Town & Country Convention Center
San Diego, CA
Workshop: Bioinformatics - Genomic Computing Techniques and Applications
Arabidopsis thaliana flower development was studied in this research. The above development consists of two consecutive processes – flower transition and organogenesis. Flower transition is conducted by three main signaling means: photoperiodic, automatic, gibberellin-dependent. Purpose of the research – to develop Arabidopsis thaliana Morphogenesis Control Model (AT-MCM1), effected by gene sub-network, consisting from 14 elements. In conjuction with Russian Science Academy and Glushkov Institute of Cybernetics / Ukraine / developed programme unit was implanted into computer environment in order to make the model AT-MCM1. Probable kinetics parameters value limits were estimated while developing model AT-MCM1 on the basis of the famous experimental data (Patrushev, 2000; Ashburner, 1990; Driever, Nüsslein-Volhard, 1998; Manoukian A. S., Krause, 1992). System description – ABC flower model is examined: A-class genes co-ordinate the identity of flower meristem, forming and development of sepals and petals. B-class genes are forming and developing of petals and stamens. C-class genes are involved in the process of forming and development of floral reproduction organs – stames and carpels. Arabidopsis transition to flowering scheme is observed: flowering induction switches vegetative development phase (V-phase) of sprout to the reproductive one (R-phase) and leads to the formation of inflorescence meristem (R/I – transition from rosette to inflorescence). Further apical meristem of reproductive sprout transforms into floral meristem (I/F – transition – from inflorescence to flower). Flower organs are formed from floral meristem within 12 phases. R/I and I/F-transitions are regulated by phase transition controller. There were computer experiments on researches of the dynamics of the examined genes’ nets on quantity and quality levels, which were conducted on the basis of the model of AT-MCM1. According to the above they determined stationary conditions and functioning regimes. Kinetics curves for molecular components (RNA, protein), describing dynamics of systems within transitions in stationary condition were also examined with the help of computer calculations. Four stationary conditions were discovered in AT-MCM1 model, transitional and synchronous regimes, which received biological interpretation.