Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Workshop: Aquaculture
            

GENETIC AND PHYSICAL MAPS OF TILAPIA

¹ 1. Hubbard Center for Genome Studies, Suite 400 Environmental Technology Building, University of New Hampshire, Durham, NH 03824 USA

² GenoMarASA, Oslo Research Park, Gaustadalleen 21, Oslo, 0349 Norway

We have constructed a second generation linkage map of tilapia from the F₂ progeny of an interspecific cross between O. niloticus and O. aureus. The map presently contains some 550 microsatellite and 14 gene-based markers spanning ~1500cM in 25 linkage groups. The average spacing of markers is about 3cM. We are also restriction fingerprinting 35,000 BAC clones to construct a 5x coverage physical map of the O. niloticus genome. As of August 1 we had analyzed 12,286 clones with FPC. We recovered 2300 contigs with an average of 5 clones per contig. The latest analysis of these data can be viewed on the WWW (hcgs.unh.edu). When coupled with a comparative map to the fully sequenced genomes of pufferfish and zebrafish, these genetic and physical maps will provide a rapid means for identifying genes underlying quantitative traits in tilapia.