Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Workshop: Aquaculture
            

PHYSICAL AND GENETIC MAPPING OF THE RAINBOW TROUT MHC CLASS I AND II REGIONS

¹ 1Center of Marine Biotechnology, University of MD Biotechnology Institutes, 701 E. Pratt St, Baltimore, MD. 21202

² School of Biological Sciences, P.O. Box 644236, Washington State University, Pullman, WA 99164-4236

³ USDA-ARS-NCCCWA, 11876 Leetown Road, Kearneysville, WV 25430

⁴ School of Biological Sciences Washington State University, 14204 NE Salmon Creek Ave. Vancouver, WA 98686-9600

One of the most unexpected discoveries in MHC genomic architectural research came from studies dealing with the teleost MHC. Initially discovered in zebrafish, the MHC class I and II regions are not linked. Previous segregation analysis in trout suggested that the class I and II regions are on completely different chromosomes yet definitive proof has yet to be shown. To learn more about MHC genomics in trout, we have isolated BAC clones harboring the MHC class Ia region that include the class Ia gene (UBA), PSMB 8-10 and ABCB3B. In addition we isolated BAC clones containing the MHC class II genes (DAA and DAB). BACs were then labeled and used as probes for in situ hybridization on OSU-142 metaphase chromosomes for determining the physical location of the MHC class I and II regions. Finally, polymorphisms found within the class Ia and II regions allowed for these regions to be assigned to specific linkage groups on the OSUxHotCreek genetic linkage map