Plant & Animal Genomes XI Conference Plant & Animal Genomes XI ConferenceJanuary 11-15, 2003
Town & Country Convention Center
San Diego, CA
Poster: High-throughput Methods
Two systems for DNA extraction from blood have been developed for use on the Biomek FX Instrument (Beckman Coulter Instruments) and other robotic platforms. One system starts with 200 ul of human whole blood and obtains 4-9 ug of high quality DNA. A fixed yield system starts with 60 ul whole blood and obtains 500-1500 ng DNA with an average of 1 ug. This DNA can be used withoutquantitation for PCR applications. The method starts with lysis of blood cells and simultaneous binding of DNA to MagneSil paramagnetic particles. A Magnetic plate holder (MagnaBot) captures the particles to allow waste removal and washing. After washing to remove impurities, the particles are briefly dried and the DNA is eluted in water or TE buffer. The DNA is suitable for all amplification systems, including genetic identity testing, single gene amplification and multiplex PCR.