Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Poster: Gene Isolation
            

DETECTION OF DELETED GENES IN RICE MUTANTS USING THE RICE GENECHIP GENOME ARRAY

¹ Torrey Mesa Research Institute, Syngenta Research and Technology, San Diego, CA 92121

² Department of Plant Pathology, Kansas State University, Manhattan, KS 66506

³ Department of Plant Pathology, The Ohio State University, Columbus, OH 43210

⁴ International Rice Research Institute, Manila, Philippines

An array-based reverse genetics method was applied to detect deleted genes in rice lines containing mutations in two known genes. The first was a dwarf mutant, which contains a deletion in d1, a heterotrimeric G protein. This mutant was selected from a population of gamma ray-induced mutants of the indica rice variety IR64. The second set of mutants contained deletions in the bacterial blight resistance gene Xa21. These mutations were induced by diepoxybutane and fast neutron mutagenesis of rice cultivar IRBB21. DNA from the mutants and wild type lines were hybridized to the Rice Genechip genome arrays separately. The arrays contain oligomers representing 21,000 rice genes. The Genechip arrays successfully detected both the d1 and Xa21 deleted genes. This technology will accelerate the discovery of a wide variety of agronomically important genes from deletion lines.