Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Poster: Large Insert Libraries
            

TWO NEW BAC LIBRARIES FOR STUDIES IN ANIMAL COMPARATIVE GENOMICS: PLATYPUS AND ACORN WORM

¹ Clemson University Genomics Institute, Room 100 Jordan Hall, Clemson, SC 29634

² Research School of Biological Sciences, Australian National University, Canberra ACT 2601

³ Arizona Genomics Institute, 303 Forbes Hall, Tuscon, AR 85721

Recently, the NIH has funded a large effort to construct BAC libraries for diverse animal taxa. The libraries will be used for studies in comparative genomics by various labs around the USA. Towards this end, we have constructed two new BAC libraries for platypus and acorn worm. The platypus(Ornithorhynchus anatinus)BAC library was constructed from a cell line derived from a baby male platypus. The cloning nenzyme was HindIII and average insert size is about 145 kb. The library provides a 13X coverage of the ~3,000 Mb platypus genome represented in 229,632 clones arrrayed in 384-well microtitre plates. The acorn worm (Saccoglossus kowalkevskii)BAC library was constructed from sperm cells collected from live worms. The cloning enzyme was also HindIII and average insert size is about 138 kb. The library provides a 10X coverage of the ~1,100 Mb acorn worm genome represented in 110,592 clones arrrayed in 384-well microtitre plates. Both libraries and their related resources may be ordered from the CUGI BAC/EST Resource Center web page (www.genome.clemson.edu).