THE CONSTRUCTION OF BAC LIBRARY AND BAC-END SEQUENCING (BES) OF KOREAN GINSENG

Department of Horticulture, and Genome Research Center, Chungnam National University, Daejeon, 305-764, Korea

Korean Ginseng(Panax ginseng C.A. Meyer) is medicinal herb that used for a long time in oriental civilization including Korea, and China. Korean Ginseng has the best medical effect among all kinds of ginseng in the world. The research for Korean Ginseng was usually focused on medical efficacy, on the other side, genome research for Korean Ginseng was lack. In this research, we have constructed Korean Ginseng BAC library and performed BAC-end sequencing (BES) for genome research. BAC library was constructed following the partial digestion of genomic DNA with HindĽ˛, and contained 106,368 clones with an average insert size of 98.61kb. BES from 1,631 BAC clones were analyzed. When sequence quality checked on Phred value (Ewing and Green, 1988) 20, a total of 1,088 BAC-end sequences (66.7%) were obtained, with average length of 417 bases. Detected clones for protein coding region was 56 sequences (5.1%), transposable elements was 48 sequences (39.3%). Specially, when protein coding region was analyzed, we found the category of enzyme related proteins including 21 sequences, and they occupied the most of molecular function. Gypsy type were 11 sequences and they also occupied the most of transposable elements. BES will used to develope sequence tagged site (STS) marker for STC framework and genetic mapping of Korean Ginseng.