Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Poster: Large Insert Libraries
            

CONSTRUCTION OF LARGE INSERT BAC LIBRARIES FROM A WIDEE VARIETY OF CULTIVATED PLANTS FOR GENE CLONING AND GENOME ORGANIZATION STUDIES

Unité de Recherches en Génomique Végétale (URGV-INRA), Genome organization, 2 rue Gaston Crémieux , CP 5708 , 91057 Évry cedex, France

Thre years ago, we have setted up a large insert BAC libraries facility at URGV-INRA (Unite de recherche en genomique Vegetale-INRA, France). We have now constructed several BAC libraries from more that 15 cultivated plants species. Other BAC libraries are being constructed and we have several national and international collaborations for BAC libraries construction. The most important constructed libraries include: two hexaploid wheat (Triticum aestivum) BAC libraries, several oilseed rape (Brassica napus) BAC libraries, three Grapevine (Vitis vinifera) BAC libraries (from three different cultivars)… All of these BAC libraries are at least 7 genome-equivalents and average insert sizes ranges from 150 to 200 kb (based on the library). Detailed description of the different BAC libraries as well as efficient protocols that have been developped for highthrough put BAC cloning will be described. These ressources will be used in different established gene cloning and genome organization programs.