Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Poster: Large Insert Libraries
            

MAKING BAC LIBRARIES FROM ALGAE, NON-SEED AND SEED PLANTS: FUNDAMENTAL PUBLIC RESOURCES FOR PLANT SCIENCE

¹ Department of Botany, University of Washington, Seattle, WA 98195-5325

² Purdue University,Dept Of Botany, West Layfayette, IN, 47907

³ Pennsylvania State University, School of Forest Resources, 304 Wartik Lab, University Park, PA 16801

⁴ Clemson Genome Institute, Clemson University, Room 100 Jordan Hall, Clemson, SC 29634

⁵ Arizona Genomics Institute, University of Arizona, 303 Forbes Building, Tucson, AZ 85721

The evolutionary transition to life on land and the diversification that followed required solving a series of problems such exposure to air and solar irraditation, life in a desiccating environment, etc. These challenges were met by plants with biochemical, cellular, anatomical and morphological solutions. We will provide access to the genetic basis of most of these innovations by making high-quality bacterial artificial chromosome, or BAC, libraries of 16 green plant species which are key in the evolutionary transition to land. These taxa include: Volvox carteri, Caulerpa mexicana, Mesostigma viride, Coleochaete orbicularis, Chara sp., Marchantia polymorpha, Anthoceros sp., Lycopodium lucidulum, Angiopteris evecta, Ceratopteris richardii, Marsilea quadrifolia, Amborella tricopoda, Nuphar adventa, Acorus gramineus, Liriodendron tulipfera, Mimulus guttatus. All libraries will be made available to the public for a minimal cost via Clemson University Genome Institute and Arizona Genomics Institute as soon as they are made and characterized. Key innovations and our current progress, including the timing of the planned availability of these 16 BAC libraries to the public at large, will be presented. Funded by the NSF's First One Hundred Program, 2002-2005.