Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Poster: Genome Sequencing & ESTs
            

EXPRESSED SEQUENCE TAG (EST) PATTERNS IN THE TRITICEAE

USDA ARS Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710-1105 USA

There are now well over 500,000 expressed sequence tags (ESTs) from the Triticeae tribe representing an abundance of cDNA library resources (see NCBI dbEST for latest information). The cDNA sources can be categorized based on attributes such as species, tissues, developmental stages, conditional stresses, and others. In tandem with the generation of this information there are several efforts underway to create consensus assemblies using different building methods based on programs such as phrap, CAP3/4, and d2_cluster. When addressing the EST contributions to contig assemblies, it is of interest to ask questions about how the contigs relate to the above mentioned attributes (species, tissues...), including other features such as mapped locations and metabolic pathways relative to the ESTs that were used in the initial assembly procedure. Additional challenges would be to show representation of multigene families and genome influences of polyploid species. Presented will be samples of display patterns exhibited by Triticeae contig assemblies. Additional information may be obtained from a Triticeae resource page (wheat.pw.usda.gov/wEST).