Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Poster: Genome Sequencing & ESTs
            

BIN-MAPPING IN WHEAT: TOWARD A PHYSICAL MAP OF WHEAT USING ESTS AND DELETION STOCKS

¹ USDA-ARS-WRRC-GGD, 800 Buchanan Street, Albany, CA 94710 USA

² Department of Agronomy and Plant Genetics, University of Minnesota, Twin Cities, 411 Borlaug Hall, St Paul, MN 55108-6026 USA

³ Department of Botany and Plant Sciences, University of California, 900 University Avenue, Riverside, CA 92521 USA

⁴ Department of Agronomy and Range Science, University of California, One Shields Avenue, Davis, CA 95616-8515 USA

⁵ Department of Plant Pathology, Throckmorton Plant Science Center, Kansas State University, Manhattan, KS 66506-5502 USA

⁶ Department of Crop & Soil Sciences, Washington State University, PO Box 646420, Pullman, WA, 99164-6420 USA

⁷ Department of Agronomy/USDA-ARS, University of Missouri-Columbia, 206 Curtis Hall, Columbia, MO 65211 USA

⁸ Department of Plant Sciences North Dakota State University, Loftsgard Hall, 470G, Fargo, ND 58105 USA

⁹ Department of Soil and Crop Sciences, Colorado State University, C101 Plant Sciences, Fort Collins, CO 80526 USA

¹⁰ Plant Sciences Unit, Department of Agronomy, University of Missouri-Columbia, 1-87 Agriculture Building Columbia, MO 65211 USA

¹¹ Department of Plant Breeding, Cornell University, 252 Emerson Hall, Ithaca, NY 14853-1902 USA

¹² Department of Crop & Soil Sciences/USDA-ARS, Washington State University, PO Box 646420, Pullman, WA, 99164-6420 USA

¹³ Genetic Resources Conservation Program, University of California, One Shields Ave., Davis CA 95616 USA

Because of the large size of the wheat genomes, it is unlikely that their actual base-pair sequences will be learned completely in the near future. This 4-year NSF PGRP-funded Project, now in its final year, takes an alternative strategy to realize the benefits of new techniques for discovering genes and learning their function. Some 90,000 wheat ESTs (Expressed Sequence Tags) have been generated from 43 cDNA libraries most of which were created by the Project from diverse wheat tissues produced under numerous controlled conditions. Of these, 10,000 unique ESTs are being identified and mapped to a physical location (bins, defined by deletion stock breakpoints). The sequence and bin location of these ESTs in the wheat chromosomes is publicly available, distributed by means of the website created for this Project (http://wheat.pw.usda.gov/NSF/). The results from this Project are immediately applicable to other crops, because of the close relationship of wheat to other species in the Triticeae tribe and other grass species, especially corn and rice. The diversity of experimental techniques and traits pursued in the individual laboratories collaborating on this Project have made it an ideal training ground for graduate students and postdoctoral scientists. The large pool of well-characterized and mapped unique DNA sequences, available in the public domain is an important resource for future Triticeae research and basic functional genomics research. See the Project website for current status and access.