Plant & Animal Genomes XI Conference

January 11-15, 2003
Town & Country Convention Center
San Diego, CA

Poster: Genome Sequencing & ESTs
            

SNPs ANALYSIS OF MULTIGENES IN HEXAPLOID WHEAT. I. PHOTOSYNTHESIS-RELATED GENES

¹ Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Maioka-cho 641-12, Yokohama 244-0813, Japan

² Center for Genetic Resource Information, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan

In order to investigate structure and expression patterns of multigenes encoding for rbcS and CAB in the hexaploid wheat, we analyzed bioinformatically EST data base of common wheat. Out of 122510 ESTs, 1067 and 320 members of the rbcS and CAB genes were selected by the BLAST method. Those members were classified into 22 contigs for the rbcS and 33 contigs for the CAB genes by the phrap method, representing each locus of three homoeologous genomes. The alignment of those contigs enabled us to trace the SNPs sites in each of both genes. The rbcS gene families are located on 2S and 5L of homoeologous chromosomes. SNPs haplotypes represented those major diversifications between genes located on 2S and 5L. Expression profiles of these contigs were monitored by the exression frequencies in the eleven tissues. Although high frequency of expression was observed in the seedling, some expressions were detected in the spikes and developing seeds. Since no difference of expression patterns between two major contig groups, the rbcS genes were expressed in a similar manner. The CAB gene families are located on 1L. The copy number of CAB genes inferred from the SNPs analysis (33) was almost equivalent to that from dot blot analysis. Two major groups were found by the cluster analysis. Chromosome locations of most contigs encoded from the CAB genes were still not determined. Both of CAB gene families were mainly expressed in seedling, showing similar expression patterns between two distinct CAB genes.