Plant & Animal Genomes XI Conference Plant & Animal Genomes XI ConferenceJanuary 11-15, 2003
Town & Country Convention Center
San Diego, CA
Poster: Genome Sequencing & ESTs
In order to establish the transcriptome of grapevine (cv Gewuerztraminer), we have isolated and sequenced cDNAs from different organs at various developmental stages. In contrast to plants like for example Arabidopsis thaliana, grapevine is particularly interesting as it produces berries. These contain unique proteins involved in secondary metabolism. Grapevine is also a woody plant and therefore contains the full set of genes required for wood formation and control. This work paves the way for functional studies of novel genes. Starting with 29,564 readings and following cleanup filtering for good quality sequences, 20,151 sequences were assembled using clustering and assembly technology from Paracel. Contigs and singlets sum up to a minimum of 9,432 different transcripts. These individual transcripts were analysed using Blast and Interproscan. About 60% of the sequences share significant sequence similarities (score >100) with proteins of assigned function. More than 80% of the sequences were found to be full-length genes. Using domain search algorithms (“dry approach”) we could assign putative functions to unknown genes, allowing us to functionally categorize them. We intend to use our collection of clones to study the profiling of proteins involved in secondary metabolism and fungal disease resistance/susceptibility. A large selection of our full-length genes with known function is now available for scientific research on our web page www.vitigen.de. Our searchable database can be used for sequence or protein comparisons with our grapevine data. Clones can be ordered from us at the following e-mail address: contact@vitigen.com.