Plant, Animal & Microbe Genomes X Conference Plant, Animal & Microbe Genomes X ConferenceJanuary 12-16, 2002
Town & Country Convention Center
San Diego, CA
Poster: Swine
A total of 3542 markers have been mapped on IMpRH radiation hybrid panel and submitted to IMpRH Server before 12 October 2001, whereas 757 markers only were mapped on the first generation map (Hawken et al., 1999). To take advantage of the different resolutions observed on the genetic and the RH maps, we have used Carthagene software to analyse SIMULTANEOUSLY genetic and RH data (genetic data used in this study were from MARC-USDA linkage families). For each chromosome, the framework map produced using a step-wise locus adding (SWLA) strategy was checked by various procedures (flips, simulated annealing ...) to try to find alternative better maps and to check if the difference of LOD with the second most likely map is at least of 3. Frequently, using checking procedures, we were able to identify maps demonstrating that the framework map proposed by the SWLA strategy was not a framework one. In addition, for two chromosomes, a map more likely than the one proposed as framework was identified. When the proposed map was not confirmed as a framework one, we manually edited the map to identify a map that could be considered as framework after the checking procedure. Additional markers were mapped relatively to the framework maps. Comprehensive maps were also produced. On average, the ratio between cM and cR observed on IMpRH framework map, is 1cM for 18 cR. This value is similar to the estimate proposed by Hawken et al on the first generation comprehensive map (48 % of the porcine genome not being accurately covered by established linkage groups). Data will be available on IMpRH Server for assignment of new markers relatively to these framework or comprehensive maps. Only the 5 main groups that have contributed have been indicated due the limitation to 5 contributing groups in the electronic submission form, other groups will be indicated on the poster.