Plant, Animal & Microbe Genomes X Conference Plant, Animal & Microbe Genomes X ConferenceJanuary 12-16, 2002
Town & Country Convention Center
San Diego, CA
Workshop: Abiotic Stress
Al toxicity is one of the most important factors limiting crop production on acid soils. The genetic control of Al tolerance has been extensively studied in the Triticeae and, as a result, several Al tolerance genes have been located to certain chromosome arms. Different studies have shown the presence of a gene in Triticeae homoeologous group 4. In wheat (Triticum aestivum L.), using a population of 91 recombinant inbred lines (RILs) segregating for Al tolerance, we provide a more extensive genetic linkage map of the chromosome arm 4DL based on RFLP, SSR and AFLP markers, delimiting the AltBH gene to a 5.9 cM interval between markers Xgdm125 and Xpsr914. In addition, utilizing a set of wheat deletion lines for chromosome arm 4DL, the AltBH gene was physically mapped to the distal region of the chromosome, between deletion breakpoints 0.70 and 0.86. In rye (Secale cereale L.) F6 RILs that segregated for one Al-tolerance gene, RFLP analysis showed that BCD1230 (previously mapped to 4RL chromosome arm) cosegregates with the gene. Using a combination of the AFLP and BSA techniques, we identified five AFLP markers tightly linked to the gene. Two of those markers, which cosegregated between each other, were 0.4 cM from the Alt3 locus. Another marker flanked the gene at 0.7 cM. We also developed a codominant PCR-based that cosegregated with the two AFLP markers located 0.4 cM from the gene. This PCR marker can be used directly to facilitate the construction of a high-resolution map in rye.