PAG-X  Plant, Animal & Microbe Genomes X Conference

January 12-16, 2002
Town & Country Convention Center
San Diego, CA

Poster: Large Insert Libraries
            

A SIMPLE 96 WELL METHOD FOR THE PURIFICATION OF BAC TEMPLATES.

Patrick J O'Mullan1 , David Herndon2 , Michael J Byers1

1 Princeton Separations, Inc. P.O. Box 300, Adelphia, NJ 07710
2 Amplicon Express 1610 NE Eastgate Blvd, Suite 880, Pullman, WA 99163

We have developed a simple method for purifying BAC templates in a 96 well format using a modified Ion exchange resin for nucleic acid binding. The plate can be used with a vacuum manifold or centrifuge for binding and elution. Yields average between 0.8 and 1.0 ug from a 5 mL overnight culture (Terrific Broth). High quality sequence reads of up to 700 bases have been obtained. A discussion of the protocol and the quality of results obtained using the method is presented.

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