Plant, Animal & Microbe Genomes X Conference

January 12-16, 2002
Town & Country Convention Center
San Diego, CA

Poster: Large Insert Libraries
            

INTEGRATED PRODUCTION AND DISSEMINATION OF LARGE-INSERT, ORDERED AND HIGH-QUALITY BAC AND BIBAC LIBRARIES FOR ACCELEREATED GENOMICS RESEARCH

¹ Institute for Plant Genomics & Biotechnology, SCIF Building Room 148, Texas A&M University, College Station, Texas, 77843-2123 USA

Large-insert, ordered, and high-quality DNA libraries are essential for modern genomics research. Bacterial artificial chromosomes (BACs) represent the state-of-the-art technology for such large-insert DNA library development. To facilitate genomics research, our laboratory has developed a series of technologies for BAC and plant-transformation-competent BIBAC library production, produced about 80 large-insert BAC and BIBAC libraries for many important plant, animal, insect and microbial species, and established the GENEfinder Genomic Resources at Texas A&M University (formerly, Texas A&M BAC Center). Currently, the TAMU GENEfinder Genomic Resources is fully equipped the advanced robotics and instrumentation required for BAC and BIBAC library production, maintenance, duplication, gridding, re-arraying and dissemination. It has become one of the largest BAC and BIBAC library resources centers in the world, providing BAC and BIBAC resources for numerous genome projects. Twenty-four of these libraries have been or are being utilized in the whole-genome physical mapping of rice, Arabidopsis, soybean, chicken, sorghum, wheat and cotton. The searchable whole-genome, BAC/BIBAC-based integrated physical maps of Arabidopsis, rice, soybean and chicken have been developed in our laboratory and are available on the web (http://hbz.tamu.edu - Physical Mapping). All of these BAC and BIBAC libraries, physical maps and derivatives are made available by the GENEfinder Genomic Resources to the research community, and can be readily accessed at our web site: http://hbz.tamu.edu/.