Plant, Animal & Microbe Genomes X Conference Plant, Animal & Microbe Genomes X ConferenceJanuary 12-16, 2002
Town & Country Convention Center
San Diego, CA
Poster: Genome Sequencing & ESTs
Olfaction is indispensable for life of animals including human. Many researchers, therefore, have paid much attention to odorant receptors and reported features of the receptors. However, limited studies have been done for clarification of the genes expressing in olfactory bulb, to which the signals from the odorant receptors were conveyed. The limitation may be the result of the fact that the volume of the olfactory bulb in experimental animals such as mice is too small to use for construction of cDNA library. In the present study, we have attempted to construct a full-length cDNA library from porcine olfactory bulb using oligo-capping method.@To obtain rough full-length rate of the library, 46 clones were randomly selected from the library, and subjected to sequence analysis from 5' end, followed by homology analysis against sequences registered in a Database (GenBank/EMBL/DDBJ); this revealed that the full-length rate was 76%. Then we selected 5000 clones randomly, sequenced the clones from 5' end, and performed the homology analysis against the database. This demonstrated that ca. 3000 independent sequences were identified, 430 of which were found to appear two times or more in the sequence analysis. Three hundred three sequences out of the 430 sequences showed high homology with the sequences registered in the database, whereas the remainings showed low homology with those in the database.