Plant, Animal & Microbe Genomes X Conference Plant, Animal & Microbe Genomes X ConferenceJanuary 12-16, 2002
Town & Country Convention Center
San Diego, CA
Poster: Microbial Sequencing and Genome Programs
Bacterial blight caused by Xanthomonas oryzae pv. oryzae is one of the most destructive diseases of rice in Asia. This bacterium is an ideal organism for studying plant-pathogen interactions, race differentiation and evolution. To facilitate molecular studies of this organism, we have constructed a bacterial artificial chromosome (BAC) library. The BAC library contains 750 clones with an average insert size of 107 kb representing 16 genome equivalents based on an estimated genome size of 4.8 Mb. A physical map of the genome has been constructed based on the BAC contigs using PCR screening of several genes and selected sequence tag sites from determined BAC end sequences of 700 clones. Homology search of the obtained sequence data revealed homologues of many Xylella fastidiosa genes. We found that many homologues of avr/pth gene families, which are present in many Xanthomonas species, were differently located on the genome. Progress of the on-going construction of BAC contigs will be presented. The resulting physical maps may then be used as tools in the whole genome shotgun sequencing efforts.