Plant, Animal & Microbe Genomes X Conference

January 12-16, 2002
Town & Country Convention Center
San Diego, CA

Bioinformatics: Databases
           Computer: Poster and Demo

A MICROSOFT ACCESS BASED DATABASE SYSTEM APPLIED TO THE MANAGEMENT OF COTTON PHYSICAL MAPPING DATA

¹ University of Georgia, 158 Riverbend Research Lab, Athens, GA, 30602, USA

² University of Colorado, Campus Box 334, Boulder, CO, 80309, USA

³ Cornell University, 320 Bradfield Hall, Ithaca, NY, 14853, USA

A Microsoft Access database has been developed for the management of BAC hybridization data related to our ongoing physical mapping effort in three species of cotton, including the two cultivated 'AADD' tetraploid species Gossypium barbadense (Pima S6) and G. hirsutum (Acala Maxxa and Tamcot GCNH), and the wild DD genome species G. raimondii. BAC libraries for all three species are currently being assayed using genomic and cDNA clones derived from linkage maps, and also dispersed repetitive DNA clones. The MS Access BAC data management application BACMan allows for the flexible management of hybridization data, and facilitates data tracking by indexing autoradiographs using a barcode system. Our application also provides for the deconvolution of multiplexed BAC hybridization data, and is directly linked to a visual basic application that allows for the automation of film data entry. The physical mapping data set is available online (http://www.plantgenome.agtec.uga.edu/cotton ) using Active Server Page technology which directly queries the MS Access database. This database system can be easily scaled to large or small physical mapping projects, and thus provides a low-cost alternative to more high-end Unix based database systems.