PAG-X  Plant, Animal & Microbe Genomes X Conference

January 12-16, 2002
Town & Country Convention Center
San Diego, CA

Poster: Genome Sequencing & ESTs
            

TARGETED RESISTANCE GENE MAPPING IN APPLE USING RESISTANCE GENE ANALOGUES FROM AN EST DATABASE

Susan Gardiner1 , Erik Rikkerink3 , Sarah Meech1 , Joanna Murdoch1 , Heather Bassett1 , Vincent Bus2 , Rachel Rusholme1 , Michael Cook1 , Andrew Gleave3 , Ross Crowhurst3

1 HortResearch, Palmerston North Research Centre, Private Bag 11030, Palmerston North, New Zealand
2 HortResearch, Hawkes Bay Research Centre, Private Bag 1401, Havelock North, New Zealand
3 HortResearch, Mt Albert Research Centre, Private Bag 92 169, Auckland, New Zealand

More than 50 candidate resistance genes identified from the HortResearch apple EST database (see companion poster) have been screened for linkage to 5 major pest and disease resistance genes conferring resistance to apple scab, powdery mildew and woolly apple aphid. This targeted strategy was used to obtain additional markers for resistance genes that are candidates for marker assisted selection in our apple breeding programme. Southern analysis is employed for identifying and confirming linkage and the more closely linked markers are being converted to PCR based markers (SCAR, SNP) to enable high throughput population screens. We will present results on new markers obtained from the EST database for the 5 resistance genes screened to date, including information on the resistance gene class that the linked markers belong to.

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