Plant, Animal & Microbe Genomes X Conference Plant, Animal & Microbe Genomes X ConferenceJanuary 12-16, 2002
Town & Country Convention Center
San Diego, CA
Poster: Wheat, Barley, Rye, Oat, and related
The genus Thinopyrum is composed of many highly salt tolerant species that have been successfully crossed with less tolerant durum and bread wheat. Three interstitial translocation lines with higher salt tolerance than Chinese Spring were identified from F3 progenies of the cross between the Ph inhibitor line (having chromosomes or chromosomal segments from Aegilops speltoides) and a disomic addition line AJDAj5 (carrying a pair of Thinopyrum junceum chromosomes). Two of the translocation lines are more salt tolerant than the two parental lines, both of which are more tolerant than Chinese Spring. The third translocation line is as tolerant as the parental lines. Results of RAPD assays on these lines suggest that most of the Th. junceum chromosome has been lost. Only two RAPD markers that were absent in Chinese Spring but present in both parental lines and all three translocation lines were identified. Analyses of 3,408 AFLP fragments amplified from 46 primer sets revealed that the three translocation lines differ from each other by having different number of markers of the Ph inhibitor line, addition line AJDAj5, and de novo origin. It is concluded that the two more tolerant translocation lines have accumulated alleles for salt tolerance from both parents whereas the third translocation has alleles transferred from only one parent, probably AJDAj5. These translocation lines are valuable wheat germplasm for salt tolerance breeding, as well as research materials for studying genetics and physiology of salt tolerance.