Plant, Animal & Microbe Genomes X Conference

January 12-16, 2002
Town & Country Convention Center
San Diego, CA

Poster: Gene Isolation
            

IDENTIFICATION OF COILED COIL-NBS-LRR-LIKE SEQUENCES AT THE Rps1-k REGION

¹ Department of Agronomy, Iowa State University, Ames, Iowa, IA50011

² Plant Biologgy Division, Noble Foundation, Ardmore, Oklahoma, OK73402

³ 1Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford Street, London, ON, Canada, N5V 4T3

⁴ Agriculture & Agri-Food Canada, 2585 County Road 20, Harrow, Ontario , CANADA N0R 1G0

Phytophthora root and stem rot is an important disease of soybean. It is caused by the oomycete pathogen Phytophthora sojae. A series of Rps genes confer resistance of soybean against P. sojae. We have applied a map-based cloning approach in isolating the most widely used gene, Rps1-k. We applied AFLP mapping and BAC cloning technologies in developing a BAC contig for the Rps1-k region. Screening of several BAC libraries representing over 20 genome equivalents showed that clones representing this region are highly underrepresented in the BAC libraries. Genetic and physical mapping data indicated that recombination is suppressed in this region. We have observed ~1.5 mb DNA/cM in this region. The Rps1-k region was localized to ~175 kb DNA. There are at least nine copies of coiled coil-NBS-LRR-type sequences in this region. Currently, the entire Rps1-k region is being sequenced. Complementation experiments are also being carried out.