Plant, Animal & Microbe Genomes X Conference Plant, Animal & Microbe Genomes X ConferenceJanuary 12-16, 2002
Town & Country Convention Center
San Diego, CA
Poster: Microbial Sequencing and Genome Programs
Anaplasma marginale is an intracellular pathogen of cattle. In collaboration with the Animal Disease Research Unit-USDA-ARS, Department of Veterinary Microbiology and Pathology, and College of Veterinary Medicine, Pullman, WA., Amplicon Express Inc. is sequencing the Anaplasma marginale genome. The goals of this project were to first obtain the sequence of specific genes of interest and then proceed with the sequencing of the complete genome. To facilitate this, a BAC library was constructed and specific BAC clones were identified by DNA hybridization. Then, a number of techniques were employed to identify additional BAC clones that represent the remainder of the genome. BAC fingerprinting was used to generate a genome-wide physical map. BAC end sequencing coupled with PCR screening of clone pools identified adjacent BAC clones that contained minimum overlap and would serve as the best candidates for sequencing. Randomly sheared shotgun libraries of selected BAC clones were used to produce high quality, publication – grade DNA sequence that could be used for immediate annotation and analysis. This strategy has allowed the concurrent analysis of specific regions within the genome while progressively generating the complete genome sequence.