Speakers: Jim Pease and Dilara Begum, EPICENTRE

Workshop Information:

On-Demand" Induction Of BAC Clones To High Copy Number For High-Throughput Sequencing And Fingerprinting.

BAC vectors facilitate stable cloning and maintenance of large genomic DNA fragments. However, the single copy nature of BAC DNA is a detriment to efficient high throughput sequencing and fingerprinting of the clones due to the low yield and purity of the BAC DNA obtained. EPICENTRE's CopyControlÔ BAC Cloning System greatly improves BAC cloning and BAC clone analysis by combining the clone stability afforded by single copy cloning with the advantages of high yields of higher purity DNA that can be obtained from "on-demand" induction of the BAC clones to high copy number. The CopyControl BAC cloning process reduces the time needed to construct a BAC library by 2 days or more. CopyControl BAC clones are initially selected as single copy clones to ensure their stability. Then, individual clones are grown in small volume culture and the clone copy number increased 10 - 20 fold. Minipreps of 1- 2 ml cultures produce sufficient high purity BAC DNA for sequencing, fingerprinting and subcloning.

The first half of the workshop will provide an overview of the CopyControl BAC Cloning process for constructing a BAC library. Data and practical tips for inducing BAC clones to high copy number for high yields of DNA will presented in the second half.

This workshop will be presented twice. The first presentation will be 4:00 - 5:00pm and the second presentation will 5:15 - 6:15pm.

This page last updated Monday, 11-Feb-2002 12:52:29 EST