Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 98pg1
IDENTIFICATION OF CAPSICUM ACCESSIONS WITH RAPD
PATTERNS
C. Lan* and G. Kuo** Department of Biology, Fu Jen Catholic
University, Hsinchuang, Taipei 242, Taiwan, ROC* and Asian
Vegetable Research and Development Center, Shanhua, Tainan
741, Taiwan, ROC**
Fifty arbitrary primers, each 10 nucleotides in length, were
used to amplify segments of DNAs of genomic DNAs from thirty
seven Capsicum accessions comprising of eight species C. annuum,
C. baccatum, C. chinense, C. eximium, C. frutescens, C.
microcarpum, C. praetermissum, and C. pubescens. DNA, fragments
obtained after PCR amplifications were analyzed by agarose gel
electrophoresis to reveal DNA polymorphisms in Capsicum
accessions. Generally, the fragments observed ranged from
greater than 2 kbp to as small as 100 bp with alternate banding
patterns appearing. Thirty two percent of the markers were fixed
in all Capsicum accessions, whereas 14% were specific to either
accession or species. The rest were polymorphic in either
accession or species. Nei's genetic distance analysis was used
to generate dendrogram to show genetic relationship among
accessions/species.
Return to Previous Page or Intl-PAG Homepage