PAG-I Plant Genome I Conference

Town & Country Conference Center, San Diego, CA, November, 1992.


PG-I: 92pg1

EFFICIENCY OF GMAPB (Genome Map Assisted Plant Breeding)

Ben Hui Liu and Patrick Hayes Department of Crop and Soil Science Oregon State University, Corvallis, OR 97331


Molecular marker-based linkage maps have been developed, or are under onstruction, for most plant species. The impetus for much of this work has been to increase the efficiency of applied plant breeding. We have simulated marker-assisted selection (MAS) in a self-pollinated doubled haploid population (barley) and an outcrossing haploid population (loblolly pine). Factorial combinations of the following were simulated for 500 replications: average linkage map densities (5%, 10%, and 20% recombination frequency between adjacent markers); number of known QTLs (quantitative trait loci) 1, 2, 4, and 6; average QTL additive effects in terms of number of standard deviations (0. 1, 0.3, 0.5, 1.0, 2.0, and 4.0); number of progenies (50, 100, 500, 1000, and 5000); selection intensities of 1%, 5%, 10%, 20%, and 40%; and selection schemes (individual selection, full-sib selection, and half-sib selection). Marker-assisted selection (MAS) is at least as efficient as conventional selection (CS). In most cases MAS is twice as efficient as CS and it can be up to thirty times as efficient in certain cases. Given the potential for rapidly genotyping large populations MAS should increase selection efficiency and reduce cycle time. The role of parent selection and maintenance of genetic variation in GMAPB is discussed. A computer software for GMAPB is roposed. GMAPB is demonstrated using data from the North American arley Genome Mapping Project.


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