Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 89pg1
THE USE OF RAPDs IN CHARACTERIZING THE PEA GENOME.
Kevin M. Folta, Brian K. Hoey, Kimberly A. Self and Neil O.
Polans, Department of Biological Sciences and Plant Molecular
Biology Center, Northern Illinois University, Dekalb, IL 60115.
We are constructing a detailed linkage map of pea based
upon both classical and molecular marker traits. Our rincipal
focus is to augment extant morphological, isozyme and RFLP data
with the powerful new RAPD procedure (see Williams et al. 1990.
NAR 18: 65 3 1, Welsh and McClelland 1990. NAR 18: 7213). In
our laboratory, decamer primers showing 50%-800% GC content have
produced between one and nine band fragments across a range of
pea accessions, generating substantial polymorphism in the
samples tested. The polymorphisms observed segregate in
Mendelian fashion among the F2 progeny of selected crosses in a
generally consistent and reproducible manner and are currently
being assigned to positions in an integrated pea nuclear genomic
map by linkage associations with previously mapped genetic loci.
These new molecular marker loci are being used for a variety of
genetical and evolutionary purposes in our program, including the
characterization of light-regulated genetic systems and the
cladistic analysis of the wild species and cultivars of Pisum.
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