Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 88pg1
CHARACTERIZATION OF TOMATO DNA CLONES WITH SEQUENCE
SIMILARITY TO THE HUMAN MINISATELLITES 33.6 AND 33.15.
Pierre Broun and Steven D. Tanksley, Department of Plant
Breeding and Biometry, Cornell University, Ithaca, NY 14850.
A tomato lambda genomic library was screened with the human
minisatellites 33.6 and 33-15 and 13 hybridizing clones were
characterized in detail. The size of the arrays varied between
100 bp and 3 kb. Their structure was complex, as the human core
sequence was interspersed with other elements and, in three
cases, part of a higher order tandem repeat. Two 33.6-related
sequences were microsatellite-like stretches. 10
minisatellite-related sequences could be genetically positioned
on the tomato high density RFLP map and did not show any
clustering pattern, which is in contrast to the organization in
the human genome. The average polymorphism detected by the
tomato minisatellite clones was estimated by probing a set of 10
tomato cultivars at low stringency, and the variability at two
polymorphic loci was evaluated among 180 accessions. The
fingerprints generated with tomato minisatellites were, in two
cases, more variable than 33.6 or 33.15 equivalents. In
addition, at two minisatellite loci, 4-8 alleles were detected
and showed 10-15 times more polymorphism than random RFLP clones.
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