Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 82pg1
PEDIGREE ANALYSIS USING MOLECULAR MARKERS.
Lisa L. Lorenzen 1, Sam Boutin 2, Nevin Young 2, Jim Specht 3,
and Randy C. Shoemaker 4, 1 Department of Zoology and Genetics,
Iowa State University, Ames, IA 50011, 2 Department of Plant
Pathology, University of Minnesota, St. Paul, MN 55108, 3
Department of Agronomy, University of Nebraska, Lincoln, NE
68583, 4 USDA-ARS-FCR, Iowa State University, Ames, IA 50011
Over 80% of the genetic composition of modern soybean
cultivars, from the Northern germplasm collection, can be
attributed to only 12 ancestral lines, thus limiting the
amount of diversity present. This limited number of ancestral
lines also makes it possible to trace chromosomal segments of
modern cultivars back to their origins. Seventy plant
introductions, accessions, breeding lines, and cultivars were
screened for polymorphisms using the mapped probe/enzyme
combination for over 200 RFLP markers, covering 20 linkage
groups. Over 45% of these probes showed a polymorphism
among the seventy tested lines. However, this percentage
varies with any two-by-two combination analyzed. With this
information, we can begin to compare the theoretical genetic
contribution of each parent involved in the pedigree with the
actual contribution realized after selection. This also allows
us to derive molecular pedigrees of these lines, and assists in
evaluating the chromosomal changes which take place during the
development of cultivars.
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