Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 80pg1
TARGETTED MAPPING AND LINKAGE ANALYSIS IN PEACH.
Jose Chaparro 1, Denny Werner 1, Dave O'Malley 2, and
Ronald Sederoff 2, Departments of Horticulture 1 and Forestry 2,
North Carolina State University, Raleigh, NC 27695.
A genomic map is being constructed for peach using
morphological, isozyme, and RAPD markers. To date, 65 markers
have been mapped in the 'Pillar' x NC174RL F2 family, with an
additional 70 markers to be added. Targetted mapping was done on
the red leaf (Gr) and malate dehydrogenase (Mdhl) loci using the
bulked segregant analysis technique. This study demonstrates how
genetic markers for morphological traits can be efficiently
identified in F2 breeding populations, without mapping the
entire genome. In peach and other tree species, the use of
genetic markers for early selection of traits expressed at
maturity could be especially valuable. We are tagging simply
inherited commercial traits such as nectarine, to use in variety
conversion backcross schemes. The principal factor determining
the adaptability range of tree species is the dormancy
requirement. We are interested in identifying loci involved in
dormancy, and understanding the genetic basis of this crucial
trait.
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